Femtosecond-Laser-Pulse Characterization and Optimization for CARS Microscopy.

Femtosecond-Laser-Pulse Characterization and Optimization for CARS Microscopy.

Blog Article

We present a simple method and its experimental implementation to determine the pulse durations and linear chirps of the pump-and-probe pulse and the Stokes pulse ORG BLACK EYED PEAS in a coherent anti-Stokes Raman scattering microscope at sample level without additional autocorrelators.Our approach exploits the delay line, ubiquitous in such microscopes, to perform a convolution of the pump-and-probe and Stokes pulses as a function of their relative delay and it is based on the detection of the photons emitted from an appropriate non-linear sample.The analysis of the non-resonant four-wave-mixing and sum-frequency-generation signals allows for the direct retrieval of the pulse duration on the sample and Hall or Caged Light the linear chirp of each pulse.This knowledge is crucial in maximizing the spectral-resolution and contrast in CARS imaging.